Overview
Tissue Inhibitor of Metalloproteinase 1 (TIMP1) is a regulatory protein that inhibits the activity of matrix metalloproteinases (MMPs), regulating the degradation and remodeling of the extracellular matrix (ECM). It binds to MMPs in a 1:1 ratio to suppress enzymatic activity, playing an important role in tissue regeneration and homeostasis maintenance. This recombinant TIMP1 is produced through an E. coli expression system, offering high purity and stable activity for application in a wide range of research and formulation contexts.
Applications
- MMP inhibition and ECM remodeling research
- Tissue regeneration and wound healing studies
- Extracellular matrix-related signal transduction studies
- Protease activity regulation research
- Skin structural improvement and ECM maintenance formulations
- Collagen degradation-inhibiting anti-aging products
- Skin elasticity and wrinkle improvement products
- Skin protection and regeneration formulations
Properties
| Item | Description |
|---|---|
| Product name | TIMP1 (Tissue inhibitor of metalloproteinase 1, human) |
| Manufacturing Source | Recombinant, E. coli |
| Purity | ≥ 98 %, as determined by Coomassie stained SDS-PAGE. |
| Endotoxin | ≤ 0.4 EU/μg of protein |
| Protein Content | Quantified using validated methods |
| Biological activity | IC50 ≤ 0.5 μg/mL (MMP-1 inhibition assay) |
| Predicted N terminal | Met1 |
| Molecular Weight | 20840.03 Da (185 amino acids). |
| Formulation | Sterile filtered through a 0.2 micron filtered 1X DPBS, pH 7.4. |
| Storage and Stability | Store at 2–8 °C for short-term use (1–2 weeks) and at -20 °C or below for long-term storage (> 2 weeks). Avoid repeated freeze–thaw cycles. |