Overview
Platelet-Derived Growth Factor AA (PDGF-AA) is a signaling protein and growth factor that regulates cell growth and differentiation, acting across diverse cell types. It particularly promotes the proliferation of fibroblasts and mesenchymal cells, and is involved in angiogenesis and tissue regeneration processes. This recombinant PDGF-AA is produced through an E. coli expression system, offering high purity and stable activity for application in a wide range of research and formulation contexts.
Applications
- Cell proliferation assay (NIH 3T3, etc.)
- Fibroblast and mesenchymal cell studies
- Angiogenesis and tissue regeneration research
- Growth factor signal transduction studies
- Skin regeneration and repair formulations
- Wrinkle improvement and elasticity enhancement products
- Damaged skin improvement and repair products
Properties
| Item | Description |
|---|---|
| Product name | PDGF AA (Platelet-derived growth factor AA, human) |
| Manufacturing Source | Recombinant, E. coli |
| Purity | ≥ 98 %, as determined by Coomassie stained SDS-PAGE |
| Endotoxin | < 0.4 EU/μg of protein |
| Protein Content | Quantified using validated methods |
| Biological activity | ED50 ≤ 50 ng/mL (NIH 3T3 cell proliferation assay) |
| Predicted N terminal | Met1 |
| Molecular Weight | 14436.79 Da (126 amino acids). |
| Formulation | Sterile filtered through a 0.2 micron filtered 50 mM sodium phosphate, 100 mM NaCl, pH 7.0. |
| Storage and Stability | Store at 2–8 °C for short-term use (1–2 weeks) and at -20 °C or below for long-term storage (> 2 weeks). Avoid repeated freeze–thaw cycles. |