Overview
Epidermal Growth Factor (EGF) is a representative growth factor that promotes cell growth and proliferation. It binds to EGFR to induce cell differentiation and signal transduction, and is involved in the recovery of skin and epithelial tissue. This recombinant EGF is produced through an E. coli expression system, offering high purity and stable activity for application in a wide range of research and formulation contexts.
Applications
- Cell proliferation assay (Balb/c 3T3, etc.)
- EGFR-based signal transduction research
- Cell migration and wound healing assay
- Cell growth and differentiation studies
- Skin regeneration and repair formulations
- Wrinkle improvement and anti-aging products
- Post-procedure skin recovery products
Properties
| Item | Description |
|---|---|
| Product name | EGF (Epidermal growth factor, human) |
| Manufacturing Source | Recombinant, E. coli |
| Purity | ≥ 98 %, as determined by Coomassie stained SDS-PAGE |
| Endotoxin | < 0.4 EU/μg of protein |
| Protein Content | Quantified using validated methods |
| Biological activity | ED50 ≤ 10 ng/mL (Balb/c 3T3 cell proliferation assay) |
| Predicted N terminal | Met1 |
| Molecular Weight | 6353.21 Da (54 amino acids) |
| Formulation | Sterile filtered through a 0.2 micron filtered 1X DPBS, pH 7.4. |
| Storage and Stability | Store at 2–8 °C for short-term use (1–2 weeks) and at -20 °C or below for long-term storage (> 2 weeks). Avoid repeated freeze–thaw cycles. |