Product Overview

Overview

Platelet-Derived Growth Factor BB (PDGF-BB) is a growth factor that promotes the proliferation of diverse cell types, acting on fibroblasts, smooth muscle cells, and connective tissue cells. PDGF exists as a dimer composed of A and B chains, and PDGF-BB is known as the form with the strongest cell proliferation activity. This recombinant PDGF-BB is produced through an E. coli expression system, offering stable activity and high quality for application in a wide range of research and formulation contexts.

Application Area

Applications

Research Applications
  • Cell proliferation assay (NIH 3T3, etc.)
  • Fibroblast and smooth muscle cell studies
  • Tissue regeneration and wound healing research
  • Growth factor signal transduction studies
Cosmetic Applications
  • Skin regeneration and repair formulations
  • Wrinkle improvement and elasticity enhancement products
  • Damaged skin improvement and repair products
Properties

Properties

ItemDescription
Product namePDGF BB (Platelet-derived Growth Factor BB, human)
Manufacturing SourceRecombinant, E. coli
Purity≥ 95 %, as determined by Coomassie stained SDS-PAGE
Endotoxin< 0.4 EU/μg of protein
Protein ContentQuantified using validated methods
Biological activityED50 ≤ 30 ng/mL (NIH 3T3 cell proliferation assay)
Predicted N terminalMet1
Molecular Weight12.4 kDa (110 amino acids, SDS-PAGE reducing conditions)
FormulationSterile filtered through a 0.2 micron filtered DPBS, pH 7.4.
Storage and StabilityStore at 2–8 °C for short-term use (1–2 weeks) and at -20 °C or below for long-term storage (> 2 weeks). Avoid repeated freeze–thaw cycles.
PDGF BB Activity Data